Plant cell engineering

Author: XieConghua
Publisher:
Publish Date: 2004-07-05
Features: The book consists of an introduction and ten chapters. The introduction focuses on introducing the concept of cell engineering, its historical development, its relationships with other disciplines, and its status and role in modern biotechnology. Chapter 1 introduces the basic techniques of cell engineering, with a focus on the environmental and nutritional conditions of in vitro culture. Chapter 2, starting from the theoretical foundation of cell engineering, introduces the concept of cell totipotency and the mechanisms of organogenesis under in vitro conditions, providing a theoretical understanding of the principles behind the generation, development, and application of cell engineering technology. Chapter 3 emphasizes the genetic variation and regulation under in vitro conditions, aiming to understand and scientifically analyze the life phenomena during the application of cell engineering technology. Chapter 4 specifically discusses other major plant tissue culture techniques beyond cell culture, including plant virus elimination and rapid propagation, anther and pollen culture, embryo and ovule culture, etc. Chapters 5 to 7 categorically introduce the main plant cell culture techniques that are currently widely studied and applied, including plant cell culture and the production of secondary metabolites, plant protoplast culture, and somatic hybridization. Chapters 8 and 9 respectively introduce the principles and techniques of cell engineering in the application of artificial seed production and genetic germplasm preservation in plants, which fall under the category of comprehensive applications of in vitro culture and other technologies. Chapter 10 provides an overview of animal cell engineering, focusing on the current research and applications of cell engineering in animal genetic improvement, reproduction, drug production, and stem cell culture, to help comprehensively understand the application fields of cell engineering technology.