Principles of Genetic Engineering (Volume 2) (Second Edition)

Author: Wu Naihu
Publisher:
Publish Date: 2002-04-01
Features: This book is based on the previous edition, incorporating new advancements in the field and adding substantial new content. It has been thoroughly revised and rewritten. The book consists of twelve chapters, divided into two volumes, with a glossary of genetic engineering terms and an index at the end. The book is composed of three organically linked parts. The first part discusses the theoretical and technical foundations upon which genetic engineering is established, focusing on the development of gene research and the modern concept of genes; the interdependent relationship between gene research and genetic engineering; the basic principles of major gene manipulation techniques; and the biochemical properties of nucleases related to gene cloning and their applications in DNA recombination. The second part, comprising Chapters 4 to 8, is the core of the book. It elaborates in detail the main contents of genetic engineering, including the construction, characteristics, and applications of various molecular cloning vectors; the construction of gene libraries, isolation, and identification of target genes; the expression and regulation of cloned genes, as well as the principles, methods, and examples of eukaryotic gene expression in E. coli. The third part, the final four chapters, primarily discusses the practical applications of genetic engineering. It emphasizes the research objectives, current status, and advancements in genetic engineering of higher plants and mammals, as well as the practical applications of recombinant DNA technology in several key fields such as clinical medicine, agricultural production, food industry, and chemical preparations. This book is a specialized academic monograph on genetic engineering with its own unique features, a novel system, and equal emphasis on both fundamental theory and practical applications. In terms of content arrangement, it focuses on scientific rigor, advanced knowledge, systematic organization, and clarity. It serves as an excellent reference not only for the teaching and research of genetic engineering in China but also for other biotechnologies, as well as for teaching and research in related disciplines such as molecular biology and molecular genetics. This book can be used as a teaching text for undergraduate and graduate students, as well as for teachers in the fields of biology, agriculture, forestry, and medicine. It can also serve as a reference for relevant researchers.