Molecular Biology Experiment Guide

Author: Wei Qun
Publisher:
Publish Date: 2004-04-30
Features: This book is a concise and practical molecular biology laboratory manual adapted to the current teaching and experimental investment of universities. The book is divided into two parts. The first part briefly introduces the experimental methods and theories of molecular biology, while the second part selects 17 student experiments, including the preparation and transformation of competent cells, plasmid DNA extraction and digestion, agarose gel electrophoresis for DNA detection, DNA recombination, genomic DNA isolation and extraction, yeast tRNA preparation, mouse liver tRNA synthetase preparation and tRNA amino acid acceptor activity determination, DNA sequencing, PCR gene amplification, expression of eukaryotic genes in prokaryotic cells, gene mutation and detection, protein blotting, Southern blotting, and site-directed mutagenesis. The book provides detailed descriptions of the practical operations of common methods and offers tips on important aspects of the experiments, enabling beginners to conduct work and achieve satisfactory results. Various appendices are also included at the end of the book. This book is suitable for universities and colleges, particularly those in biology, agriculture, forestry, and medicine, to use as a teaching manual for molecular biology experiments. It can also serve as a reference for relevant researchers and high school biology teachers.