Laser scanning confocal microscopy technology

Author: Wang Chunmei / Huang Xiaofeng / Yang Jiaji / Chen Zhinan (Editor-in-Chief)
Publisher:
Publish Date: 2004-07-01
Features: The book is divided into four parts and twenty-nine chapters. Part I introduces the basic principles of laser scanning confocal microscopy technology, including fundamental techniques for image acquisition, image processing, and dynamic fluorescence measurement. Part II focuses on the specific applications of laser scanning confocal microscopy. It provides detailed discussions on calcium ion transport, calcium store determination, calcium ion regulation mechanisms in secretion phenomena, and the molecular biology of calcium channel activation. Additionally, it introduces methods for pH measurement, calcium ion measurement, echinoderm fertilization imaging, live-cell confocal imaging of intact embryos, and fluorescence in situ hybridization, as well as the analysis of gene and protein expression in fruit flies. To facilitate experimental operations for researchers, Part III briefly covers fluorescent probes and staining techniques used in laser scanning confocal microscopy, as well as histological techniques and immunofluorescence cytochemistry and affinity histochemistry closely related to confocal microscopy applications. Part IV explores the applications of laser scanning confocal microscopy technology in plant research. The appendix introduces commonly used reagents and specific preparation methods for immunofluorescence cytochemistry. Beyond explaining the basic principles, the book emphasizes practicality, providing detailed descriptions of key considerations in experiments. Special attention is given to the inclusion of over 40 color images showcasing the real-world applications of confocal microscopy across various fields, designed to delight and inform the readers.