Genetically Modified Animal Technology Manual (Second Edition) (Second Edition)

Author: Karl
Publisher:
Publish Date: 2004-09-01
Features: The original edition of this manual is the first manual jointly compiled by globally renowned laboratories on animal transgenesis basic techniques. It is richly illustrated and introduces the genetic transfer of various important laboratory and domestic animal species. The technical methods described in this manual are adopted by leading laboratories worldwide. This is the second edition, which updates 80% of the content based on the previous edition and adds techniques widely used in animal model systems, such as microinjection and ES cell nuclear transgenic engineering technology. To provide readers with more information and facilitate research, this manual comprehensively provides a database and online resources for current transgenic animal technologies, as well as a detailed explanation of the nomenclature of transgenic animals. Transgenic animal technology and the techniques for introducing functional genes into animals are powerful tools for analyzing complex biological processes. The problems addressed span scientific fields such as biomedical, biological applications, and agricultural production. Transgenic methodologies continue to evolve and have become an interdisciplinary field, regarded as a tool for expanding gene expression, regulation, and function. There are many reviews on transgenic animals, which closely follow research trends and are highly helpful to researchers. However, in this field, apart from manuals on mouse embryology, no manual compiling the methods adopted by globally renowned laboratories in their respective disciplines was published before the release of this edition. This manual covers all aspects of animal gene transfer techniques, providing detailed discussions on various species hosts from the molecular to the individual level. In this sense, this manual will serve as a bridge between researchers and become a training textbook for students and technicians dedicated to the development of transgenic animal model systems.