Modern molecular biology experimental techniques

Author: Lu Shengdong, Editor-in-Chief
Publisher:
Publish Date: 1999-12-01
Features: This book systematically and comprehensively introduces common experimental techniques and methods in modern molecular biology. The book is divided into 32 chapters. The introduction provides an overview of major advancements in genetic engineering and molecular biology, while the other chapters cover topics such as routine laboratory equipment and techniques in molecular biology laboratories, isolation and purification of nucleic acids, labeling of nucleic acid molecular probes, nucleic acid molecular hybridization, gene cloning techniques, genomic libraries, cDNA libraries, DNA sequencing and its automation, expression of foreign genes in prokaryotic cells, expression of foreign genes in eukaryotic cells, large-scale culture of genetic engineering bacteria, protein refolding and purification from engineered bacteria, large-scale culture of animal cells, polymerase chain reaction (PCR) technology, chemical synthesis of DNA, solid-phase synthesis of peptides, amino acid composition and sequence determination of proteins, regulation of eukaryotic gene expression, transgenic animals, transgenic plants, human gene therapy, in vitro and in vivo transgenic methods, non-viral vector gene therapy, research methods for apoptosis, microsatellite technology, rapid amplification of cDNA ends (RACE), mRNA differential display, DNA chip technology, and common genetic inheritance statistical analysis methods. The book emphasizes the rigor, practicality, and reliability of methodologies. It can serve as a reference book for graduate students in disciplines such as science, engineering, agriculture, medicine, pharmacy, forestry, animal husbandry, environmental science, marine science, and food science, as well as for researchers in various fields of life sciences.