Advanced Biochemistry Experiment Tutorial

Author: Wang Chongqing
Publisher:
Publish Date: 2001-07-01
Features: This book is compiled from experimental teaching materials of the Department of Biology at Peking University. It includes 14 experiments, covering techniques and methods for the separation, purification, and analysis of biomolecules such as proteins, enzymes, and nucleic acids. Examples of experiments and techniques include the preparation of crystalline enzymes, isoelectric focusing electrophoresis to determine the isoelectric point of proteins, and affinity chromatography for the purification of trypsin, among others, to meet the needs of biomolecule research. To adapt to the development of molecular biology, experiments such as the isolation and purification of plasmid DNA, the identification of plasmid DNA, molecular hybridization of plasmid DNA, and the preparation and transformation of competent cells have been included. Additionally, immunological chemistry experiments such as immunoelectrophoresis and ELISA detection methods have been incorporated. Before each experiment, the principles are concisely and clearly explained in an easy-to-understand manner. Following each experiment, a "Discussion of Results and Precautions" is specially written, summarizing the accumulated experience of instructors from years of teaching and research. The "Thought Questions" and "Answers" also help further master the experiments. This book is suitable as a laboratory manual for undergraduate students of comprehensive universities, normal universities, medical schools, and agricultural universities, as well as for non-biochemistry graduate students in biology departments. It can also serve as a reference for researchers engaged in biochemistry and molecular biology research.