Plant Cell Engineering Experimental Technology (Biological Laboratory Series)

Author: Sun Jingsan
Publisher:
Publish Date: 2006-04-24
Features: This book comprehensively discusses the fundamental theories, experimental techniques, and application achievements in various branches of plant cell engineering. It particularly focuses on the principles of experimental design, key techniques, and operational precautions. The book is divided into twenty-two chapters, covering topics such as the equipment and basic operations of plant cell engineering laboratories, the composition and preparation of culture media, cell culture, protoplast culture and somatic hybridization, callus induction and plant regeneration, plant organ cloning, embryo culture and endosperm culture, in vitro fertilization and zygote culture, haploid plant induction, anther and isolated microspore culture, somatic cell non-sexual variation and plant improvement, artificial seeds, plant virus elimination and micropropagation, rapid breeding based on in vitro culture, regulation techniques and large-scale cultivation of plant cells producing useful secondary metabolites, in vitro storage of plant germplasm, microalgal cell culture, plant insertional mutation library technology, Agrobacterium-mediated gene transfer, direct transfer of exogenous genes, identification techniques of transgenic plants, cytological examination of plant tissue cultures. Each chapter introduces specific experimental procedures, and the appendix at the end of the book provides formulations of commonly used culture media. The book is highly practical and serves as a teaching resource for faculty and students in plant, horticulture, and biotechnology programs at universities. It is also suitable for researchers, technicians, and laboratory personnel in related fields.