From Gene to Genome: Concepts and Applications of DNA Technology

Author: JW Dell [USA]
Publisher:
Publish Date: 2004-01-08
Features: The rapid development of gene technology, genetic engineering, recombinant DNA, and gene cloning has pushed molecular biology to the forefront of life sciences. This book provides a concise and clear explanation of the principles and applications of core molecular biology techniques. It begins with a brief introduction to the fundamental concepts of molecular biology, then introduces key molecular biology methods and their integrated use, ranging from the cloning and study of individual genes to the sequencing of entire genomes and the analysis of genomic information. Finally, the book also explores the applications of these technologies in biotechnology, medicine, agriculture, and the broader field of life sciences research. The content covers molecular biology fundamentals, gene cloning, purification and separation of nucleic acids, DNA cutting and ligation, vectors, genomic libraries and cDNA libraries, polymerase chain reaction, DNA sequencing, sequence data analysis, gene variation analysis, gene expression analysis, gene function analysis, medical applications, genetic engineering, and more. This book is suitable for undergraduate and graduate students, as well as teaching and research personnel in related disciplines and fields such as biochemistry, molecular biology, genetics, cell biology, biotechnology, and bioengineering.