Practical Molecular Biology Techniques

Author: Zhao Xiaoyu
Publisher:
Publish Date: 2006-06-01
Features: The content of this book is centered on genetic recombination, integrating basic research methods from genomics, proteomics, and molecular systematics. It introduces fundamental principles and specific experimental protocols, with particular emphasis on easily overlooked issues in experimental operations. Although many well-established companies have developed a series of kits, which bring convenience to researchers performing specific operations, the negative consequences often include difficulty in troubleshooting and analysis. This is especially true for beginners, leading to over-reliance on reagent companies, which is not ideal for researchers dedicated to specialized studies. From another perspective, a clear understanding of the principles behind the methods and techniques used will help operators use kits more effectively. In reality, molecular biology experiments are highly experiential, making the accumulation and absorption of experimental experience essential. The book also cites several representative research papers for reference and introduces the special concepts behind new product developments by renowned molecular biology reagent companies, providing concrete examples to broaden readers' perspectives. Necessary common data are included to enhance the book's practicality. To aid readers' comprehension, examples or references are listed in each chapter, with references primarily in Chinese (all obtainable from the China National Knowledge Infrastructure database), facilitating understanding and retrieval. For deeper exploration, readers can further search for original materials based on the references cited in these papers. The book comprises 12 chapters, with content centered on genetic recombination techniques, introducing fundamental molecular biology experimental techniques, including gene cloning operations, DNA library construction, polymerase chain reaction (PCR) technology, DNA sequencing, molecular markers, and hybridization. It also covers genetic diversity analysis techniques in molecular systematics research, eukaryotic gene expression regulation, and basic proteomics research methods, as well as major alignment analysis techniques, common databases, and software in bioinformatics. This book is suitable for graduate students in various life science disciplines and can also serve as a reference for teachers and researchers engaged in related biotechnology work. The book is centered on genetic recombination techniques, introducing fundamental molecular biology experimental techniques, including gene cloning operations, DNA library construction, and polymerase chain reaction technology.