Modern Practical Histology and Histochemical Techniques

Author: Liu Nengbao, Wang Ximing, Editors
Publisher:
Publish Date: 2003-03-01
Features: The book includes 7 chapters, introducing various modern histological and histochemical techniques.
Chapter 1, Tissue Culture: Introduces basic tissue culture techniques, culture media, passaging of cells, cell cloning techniques, cultivation of various organs and tissues, and the application of tissue culture techniques.
Chapter 2, Histochemistry: Introduces the basic concepts of classical histochemistry, the fundamental requirements of histochemical techniques, specimen preparation, multiple light and electron microscopic histochemical techniques, primarily the light and electron microscopic histochemical techniques of various enzymes.
Chapter 3, Immunohistochemistry: Introduces the basic concepts of immunohistochemistry, the fundamental requirements of immunohistochemical techniques, the concepts of antigens and antibodies, the principles of various basic immunohistochemical techniques, including immunofluorescence techniques, immunoenzyme techniques, avidin-biotin techniques, and immunogold colloid techniques. Specimen preparation and multiple staining procedures, including single and double-labeled light and electron microscopic immunohistochemical staining procedures.
Chapter 4, In Situ Hybridization Histochemistry: Introduces the basic techniques of in situ hybridization, various operational procedures, dual-labeling procedures combining in situ hybridization and immunohistochemistry, dual in situ hybridization labeling procedures, thin-section and whole-tissue in situ hybridization procedures, and electron microscopic in situ hybridization procedures.
Chapter 5, Quantitative Histochemical Techniques: Introduces subjective quantitative methods, microscopic spectrophotometry, and image analysis systems.
Chapter 6, Flow Cytometry and Laser Scanning Confocal Microscopy: Introduces the working principles, functions, specimen preparation, and various staining methods of flow cytometry, as well as the instrument system composition, working principles, main characteristics, basic functions, and several fluorescent staining methods for specimen preparation of laser scanning confocal microscopy.
Chapter 7, Detection of Apoptotic Cells: Introduces the light and electron microscopic morphological detection of apoptotic cells, flow cytometry detection, nJNEL detection, detection of apoptosis-related proteins and enzymes, and DNA lysis detection. The numerous modern histological and histochemical techniques introduced in this book can serve as references and applications for teachers, physicians, graduate students, and researchers engaged in basic research and clinical diagnosis.