Protein Two-Dimensional Electrophoresis Experiment Manual

Author: Ni Yidong
Publisher:
Publish Date: 2006-01-01
Features: This book is a practical guide on the experimental methods of two-dimensional electrophoresis. It begins by introducing the principles of two-dimensional electrophoresis, the development of experimental techniques, and the use of electrophoresis equipment and related accessories. Focusing on practice, it emphasizes the experimental operations of isoelectric focusing using the Multiphor II system as a carrier, as well as the techniques and specific applications of the second-dimensional SDS-polyacrylamide gel electrophoresis in both vertical and horizontal formats. Additionally, it explores different protein sample preparation methods for various research subjects and their impact on two-dimensional electrophoresis results. This book is a summary of the author's practical experience and lessons, offering strong practicality, guidance, and operability. It serves as an excellent reference for professionals engaged in two-dimensional electrophoresis techniques. The book is suitable for laboratory staff in biochemistry, molecular biology, biotechnology, medical and health fields, as well as researchers and educators in related disciplines such as agriculture, forestry, and animal husbandry. The main contents of the book include: an overview of two-dimensional electrophoresis, protein sample preparation, the direction of IPG-Dalt two-dimensional electrophoresis—IPG single focusing, experimental results, and discussions.