Principles and Methods of Biochemical Experiments

Author: Li Jianwu
Publisher:
Publish Date: 2000-04-01
Features: The book is divided into three parts: Basic Principles of Biochemical Experiments, Experiments, and Appendices. It focuses on discussing the fundamental principles of various common biochemical experimental methods, including chromatography, electrophoresis, spectrophotometry, and the preparation of biopolymers. The experimental section includes 61 selected experiments, covering techniques for the separation, preparation, analysis, and identification of carbohydrates, lipids, proteins, nucleic acids, enzymes, hormones, and vitamins, as well as the Waring blender method. Among these, some traditional experiments that effectively strengthen students' basic experimental methods and skills are retained, while new biochemical experimental techniques developed recently are also introduced. These experiments have been repeatedly verified through years of teaching practice and scientific research, proving to be relatively mature. Most experiments can be completed within 8-16 hours. Some experiments can be combined to form a comprehensive experiment, or conducted independently as separate experiments, facilitating teaching arrangements. References are provided after each experiment. The appendix includes various commonly used data tables and instructions for operating common instruments, all available for readers to consult. This book is intended for undergraduate students in comprehensive universities, normal universities, medical schools, and agricultural and forestry colleges, as well as other biochemical experimental technology workers. It can serve as a textbook for biochemical laboratory courses in higher education institutions and also as a reference tool for small-scale experimental techniques.