Patch clamp experimental technique

Author: Chen Jun
Publisher:
Publish Date: 2005-04-01
Features: The patch-clamp experimental technique has revolutionized physiological research at the cellular and molecular levels. The two pioneers of this technology (Neher and Sakmann) were awarded the Nobel Prize in 1991. This book systematically and comprehensively introduces the basic principles, experimental procedures, and practical applications of this technique, including: the basic principles and operation of patch-clamp technology, whole-cell recording, analysis of pump currents and transport currents, intracellular perfusion, giant cut patch-clamp, tissue slice patch-clamp technology, brainstem and spinal cord specimen patch-clamp, application of caged compounds, simultaneous measurement technology combining calcium fluorescence photometry and patch-clamp, as well as 13 topics such as ion channel noise analysis, analysis of single ion channel recording data, membrane capacitance measurement, and lipid planar membrane methods applied in ion channel research. The text is rich in concise introductions and skillful insights, complemented by illustrations and practical content. This book is suitable for professionals in physiology, neuroscience, cell biology, and related fields as an introductory guide and reference for mastering patch-clamp technology.