Recombinant antibody

Author: Shen Beifan
Publisher:
Publish Date: 2005-05-01
Features: Receptor antibody technology enables the production of humanized antibodies and human antibodies, which cannot be achieved through conventional polyclonal or monoclonal antibody preparation methods. This book is divided into 22 chapters and introduces four aspects of technology to readers: antibody library technology, including combinatorial antibody libraries, phage antibody libraries, bacterial and yeast display antibody libraries, ribosome display antibody libraries, and antibody library screening techniques; genetic modification and expression of receptor antibody genes, including antibody affinity maturation, antibody humanization, and stability modification, as well as the expression of antibody genes in prokaryotes, yeasts, insects, mammalian cells, plants, and transgenic animals; improving the effector functions of receptor antibodies, involving antibody binding with isotopes, drugs, and toxins for "guided drugs," bispecific antibodies, antibody-enzyme fusion proteins, immunocytokines, and intracellular antibodies; antibody specificity analysis and identification, primarily introducing antibody isolation and purification, antibody labeling techniques, antibody affinity determination, antibody epitope analysis, antibody gene sequencing and structural modeling, as well as common antibody identification techniques such as ELISA, immunohistochemistry, FACS analysis, immunoprecipitation, and Western blot. This book covers most of the current technologies in the preparation and modification of receptor antibodies, making it a valuable reference for technical personnel engaged in antibody research, biotechnology companies, and students and faculty of relevant universities and colleges.