Gene Expression Sequence Tag (EST) Data Analysis Manual: Data Analysis Manual

Author: Hu Songnian
Publisher:
Publish Date: 2005-05-01
Features: This manual takes the processing and analysis workflow of Expressed Sequence Tags (EST) as the main line, combined with numerous examples, to systematically introduce EST sequencing, the construction of EST data analysis platforms, literature retrieval and database queries for EST, EST sequence clustering and assembly, functional classification of EST, metabolic pathways, and interpretation of gene products, among other methods and commonly used software, in a "follow me" style. Additionally, it explains how to utilize a large number of online resources and professional software to analyze the basic characteristics and functions of genes and their protein products at the gene and protein levels. At the nucleotide level, it covers open reading frame identification, gene function prediction, gene structure analysis, alternative splicing analysis, gene polymorphism site analysis, gene expression regulation region analysis, statistical analysis of sequence GC content and codon usage bias, as well as restriction enzyme site identification and primer design. At the amino acid level, it includes analysis of physicochemical properties, domains, secondary structure, tertiary structure, and phylogenetic relationships. The book systematically introduces EST sequencing, the construction of EST data analysis platforms, literature retrieval and database queries for EST, EST sequence clustering and assembly, and functional classification of EST.