Environmental Biology Experiment Technology

Author: Zhang Qingmin
Publisher:
Publish Date: 2005-05-01
Features: This book is divided into 6 chapters. Chapter 1 introduces the main objectives and requirements of the experimental technology course; Chapter 2 covers basic experimental techniques that students must firmly master; Chapter 3 discusses modern biological experimental techniques; Chapters 4 to 6 focus on specialized experimental techniques, specifically environmental microbiology, environmental ecology, and bioavailability experimental techniques. This book encompasses all experimental techniques in environmental biology and serves as an experimental textbook for students of environmental science and related disciplines. It provides a solid foundation in experimental techniques for students pursuing different degrees and is also a practical reference for scientists engaged in environmental science experimental research.
Table of Contents
Table of Contents
Chapter 1 The Objectives and Requirements of the Experimental Technology Course
1.1 The Objectives of Experimental Technology
1.2 Basic Requirements for the Experimental Technology Course
1.3 Laboratory Work Records
1.4 Research Projects
1.5 Writing Experimental Reports and Papers
Chapter 2 Basic Experimental Techniques
2.1 Measurement of Liquids
2.2 Preparation of Solutions
2.3 Use of Balances
2.4 Water, pH, and Buffer Solutions
2.5 Determination of Dissolved Oxygen
2.6 Measurement of Conductivity
2.7 Measurement of Turbidity
2.8 Determination of Absorbance
2.9 Samples and Sampling
2.10 Experimental Design
Chapter 3 Modern Biological Experimental Techniques
3.1 Cell Culture Techniques
3.2 Study of Animal and Plant Tissues and Cells
3.3 Genetic Engineering Techniques
3.4 Immunological Techniques
3.5 Centrifugation Techniques
Chapter 4 Environmental Microbiology Experimental Techniques
4.1 Preparation of Culture Media
4.2 Sterilization and Disinfection
4.3 Microbial Inoculation Techniques
4.4 Observation of Microbial Morphology
4.5 Bacterial Staining and Observation
4.6 Detection of Bacterial Counts in Water
4.7 Detection of Coliforms in Water
4.8 Detection of Microorganisms in Soil and Air
4.9 The Role of Microorganisms in the Nitrogen Cycle
4.10 Cultivation of Photosynthetic Bacteria and Their Purification of High-Concentration Organic Wastewater
4.11 Isolation and Characterization of Phenol-Degrading Bacteria
4.12 Microscopic Observation of Microorganisms in Activated Sludge and Enumeration of Microscopic Animals
4.13 Determination of Oxygen Consumption Rate in Activated Sludge, Biochemical Treatability, and Toxicity of Wastewater
4.14 Isolation of Surfactant-Degrading Bacteria
Chapter 5 Environmental Ecology Experimental Techniques
5.1 Methods for Compiling Life Tables and Fecundity Tables
5.2 Logistic Growth of Populations in Limited Environments
5.3 Effects of Temperature on Fish Respiration Rate
5.4 Determination of Primary Productivity in Water Bodies
5.5 Determination of Secondary Productivity
5.6 Measurement of Species Diversity Indices in Communities
5.7 Survey of Quantitative Characteristics of Plant Communities (Drawing Species-Area Curves)
Chapter 6 Bioavailability Experimental Techniques
6.1 Seed Germination Toxicity Test
6.2 Vicia faba Micronucleus Test
6.3 Algal Acute Toxicity Test
6.4 Daphnia Acute Toxicity Test
6.5 Determination of Dehydrogenase Activity
6.6 Determination of Carboxymethylcellulase Activity
6.7 Effects of Pollutants on Cytochrome C
6.8 Effects of Pollutants on Acetylcholinesterase
6.9 Toxicity Determination of Pollutants on Zebrafish Embryonic Development
6.10 Bio Experiment of Environmental Pollutants—Bioluminescence Photometry of Luminescent Bacteria
6.11 Ames Mutagenicity Test
Appendix 1 SI Units and Their Applications
Appendix 2 Experimental Errors and Methods to Improve Experimental Accuracy
Appendix 3 Reagents That Are Prone to Degradation and Require Special Storage Methods
Appendix 4 Common Chemical Sterilization Agents
Appendix 5 Common Stains in Biology and Their Characteristics
References