Biological Separation Engineering

Author: Sun Yan
Publisher:
Publish Date: 2005-03-01
Features: This book focuses on the separation and purification techniques of biomolecules, systematically introducing the fundamental principles, separation operations, process theory, and applications of bio-product separation and purification. The content is divided into 12 chapters, including Introduction, Cell Separation and Disruption, Primary Separation, Membrane Separation, Extraction, Adsorption Separation Technology and Theory, Liquid Chromatography, Affinity Chromatography, Electrophoresis and Electrochromatography, Protein Refolding, Crystallization, and Drying. Among these, Chapters 2 and 3 primarily introduce the pretreatment of bio-separation processes and primary separation techniques such as precipitation fractionation and foam separation; Chapter 4 covers various membrane separation methods, their characteristics, and their applications in bio-separation; Chapter 5 discusses various extraction methods, particularly biphasic aqueous extraction and reverse micellar extraction techniques, which are applicable to the separation and purification of biomolecules; Chapters 6 to 10 elaborate on the fundamental principles, characteristics, basic theories, and applications of core bio-separation processes such as adsorption (including ion exchange), chromatography, electrophoresis, electrochromatography, and protein refolding. These chapters include recent research advancements in the field, forming the core of the book; the final two chapters introduce the fundamental theories of crystallization and drying and their applications in bio-separation. This book is primarily intended as a textbook for undergraduate and graduate students in bioengineering, biochemical engineering, and related disciplines at universities and colleges. It can also be used as a reference for researchers, technicians, and management personnel engaged in biotechnology, biochemical engineering, and biopharmaceutical research.