Mouse developmental biology and embryonic experimental methods

Author: Jin Yan
Publisher:
Publish Date: 2005-01-01
Features: This book is divided into 2 parts, totaling 25 chapters. Part 1 includes 9 chapters, focusing on introducing the relevant concepts, general processes, and fundamental principles of developmental biology, as well as an overview of mouse embryonic development. It provides a detailed elaboration on key issues in developmental biology, such as model systems and body plan patterns, cell differentiation, morphogenesis and organ formation, regulation of embryonic growth and development, and post-embryonic development. Additionally, it covers the development of the nervous system, limbs, skeleton, muscles, blood vessels, craniofacial and oral structures, and offers an overview of stem cells, which are a rapidly advancing research area. Part 2 includes 16 chapters, detailing various techniques and methods for mouse embryonic experiments, including experimental injuries in fertilization and embryo transfer, as well as gene transfection techniques. It emphasizes how to prepare transgenic mice, gene knockout mice, and chimeric mice, and provides systematic methods for analyzing these mice and experimental results, including reporter genes, cell markers, karyotype analysis, and chromosomal integration gene localization.