PCR latest technology principles, methods and applications

Author: Huang Liuyu
Publisher:
Publish Date: 2005-01-01
Features: The editors of this book are a group of people who almost deal with PCR every day. They combine theory with practice, providing both concise principles and practical operation steps and application examples for each PCR method, as well as summaries of experience in handling difficult problems. It is a rare operational manual for PCR technology. Its comprehensiveness and practicality make it a reference tool. As a molecular biologist, keeping this book on the desk or next to the experimental bench, whether for reading or reference, is believed to be beneficial in improving the quality and efficiency of scientific research and promoting the development of science and technology. — Huang Cuifen, Academician of the Chinese Academy of Engineering
This book nearly collects all PCR methods that can beed so far, categorized according to the purposes and applications for which these PCR methods were designed, into fourteen chapters. The content includes reverse transcription PCR, PCR for amplifying unknown sequences, mutation PCR, quantitative PCR, immune PCR, the combination of PCR with other methods, and the application of PCR in various biological gene typing, genetic disease diagnosis, forensic medicine, and tumor diagnosis. Each type of PCR method is briefly introduced with its principles, emphasizing specific operation steps and application examples, as well as summaries of experience in handling difficult problems. For the convenience of readers to trace and retrieve, each method is accompanied by detailed references. At the end of the book, a subject index on methods and applications is compiled for quick retrieval of relevant keywords. This book is experimentally application-oriented, with various PCR methods categorized for readers to retrieve and consult targetedly according to different experimental purposes, making it convenient and practical. It is available for scientific researchers or teaching personnel engaged in molecular biology, biochemistry, genetics, immunology, molecular epidemiology, forensic medicine, cell biology, medicine, and life sciences, serving as a reference tool for biological and medical-related laboratories.