Genetic engineering

Author: Chen Yongqing
Publisher:
Publish Date: 2003-03-27
Features: This book is a specialized monograph that systematically elaborates on the fundamental theories and practical techniques of genetic engineering, reflecting the latest advancements in the field. The book consists of fourteen chapters, organized into three organically connected parts. The first part primarily introduces basic theoretical concepts, including an overview of molecular genetics in prokaryotes and the characteristics of eukaryotic genes. The second part mainly covers genetic engineering operational techniques, including fundamental methods of recombinant DNA technology, polymerase chain reaction (PCR), gene cloning and isolation, genomic engineering, strategies for high-efficiency expression of genes in Escherichia coli, and the expression of foreign genes in yeast and mammals. The third part introduces the practical applications of genetic engineering, including transgenic animals, transgenic plants, microbial genomics, gene chip technology, and human genome research. The book emphasizes both theory and practice, with content arrangements that prioritize scientific rigor, systematic organization, logical clarity, and practicality. It serves as a reference for researchers in the field of biology and for faculty and students at universities and colleges.