Patch-clamp technique and its applications

Author: Kang Huaguang
Publisher:
Publish Date: 2003-01-01
Features: This book is supported by the Excellent Research Achievement Monograph Publication Fund of the National Natural Science Foundation of China. It is a summary of the author's research work in the field of "Patch-Clamp Technology and Its Applications," incorporating advanced international technologies and recent research findings. The content includes: cell electrophysiology and patch-clamp technology, the assembly of patch-clamp systems and an overview of experimental techniques, the principles of patch-clamp amplifiers and low-noise design, single-channel and whole-cell current recording techniques, data acquisition and analysis, membrane capacitance monitoring and amperometric measurement techniques for cellular secretion activities, measurement of intracellular calcium ion concentration and calcium store characteristics, patch-clamp technology on brain slices, pharmacological properties of cardiomyocytes, and ion channel characteristics of plant cells. Additionally, some content in the book has been used as teaching material in the postgraduate programs of biomedical engineering and biophysics at Huazhong University of Science and Technology, achieving good results. This book can serve as a textbook for graduate students, senior undergraduate students, and researchers in universities, colleges, research institutes, and related courses such as cell electrophysiology and biophysics. It is also a valuable reference for scientists and researchers in the field of scientific research.
The book is supported by the Excellent Research Achievement Monograph Publication Fund of the National Natural Science Foundation of China. It is a summary of the author's research work in the field of "Patch-Clamp Technology and Its Applications," incorporating advanced international technologies and recent research findings. The content includes: cell electrophysiology and patch-clamp technology, the assembly of patch-clamp systems and an overview of experimental techniques, the principles of patch-clamp amplifiers and low-noise design, single-channel and whole-cell current recording techniques, data acquisition and analysis, membrane capacitance monitoring and amperometric measurement techniques for cellular secretion activities, measurement of intracellular calcium ion concentration and calcium store characteristics, patch-clamp technology on brain slices, pharmacological properties of cardiomyocytes, and ion channel characteristics of plant cells. Additionally, it covers low-noise measurements, signal acquisition, analysis, and processing, fluorescence measurements, and imaging techniques for cells and tissues. These topics span various fields of life sciences and information science, such as physiology, pharmacology, cell biology, neurobiology, endocrinology, and plant cell physiology.