PCR Technology Experiment Guide (Molecular Cloning Experiment Guide Series.)

Author: Di
Publisher:
Publish Date: 2004-01-01
Features: Since the invention of Polymerase Chain Reaction (PCR), it has undergone continuous development and improvement over the past 20 years and is now widely used in various fields. It has become a routine technique in laboratories for molecular biology, clinical diagnostics, forensic testing, and more. This book is a photocopied version of the second edition of PCR Primer: A Laboratory Manual published by Cold Spring Harbor Laboratory Press in the United States. It is a comprehensive update and upgrade of the edition. The book is co-authored by experts in PCR research, making it a new and authoritative experimental manual on PCR technology. Its content covers everything from simple PCR operations to new technical improvements, from basic PCR techniques to various creative PCR application techniques, leaving readers amazed and inspired. Specific topics include PCR introduction, sample preparation, primer design, PCR product detection (quantification and analysis), PCR for RNA samples, PCR-mediated cloning, PCR-based mutation preparation, and other amplification techniques. Each procedure is accompanied by troubleshooting tips and complete references for clarification and research. This book is the sister volume to "Molecular Cloning: A Laboratory Manual" and serves as a reference for researchers and educators in the fields of molecular biology, genetics, cell biology, biochemistry, and various medical disciplines. It is not only a tool for the laboratory but also an essential classic for researchers to broaden their horizons and expand their thinking. The book includes topics such as PCR introduction, sample preparation, primer design, PCR product detection (quantification and analysis), PCR for RNA samples, PCR-mediated cloning, PCR-based mutation preparation, and other amplification techniques.