Application of gene cloning technology in pharmaceuticals

Author: Zhang Chaoying
Publisher:
Publish Date: 2004-01-01
Features: This book combines theory with examples, reflecting cutting-edge science and being highly practical. It is suitable for technical personnel engaged in biotechnology and pharmaceutical research and production, as well as students and teachers in related fields at universities and colleges. The book discusses the fundamental principles of gene cloning technology and its research and applications in biopharmaceuticals. The book is divided into two parts: Technology and Application. The Technology part emphasizes systematic discussions on the basic knowledge of gene cloning technology, including: an overview of gene cloning technology, commonly used tools for gene cloning in pharmaceuticals, commonly used plasmid and phage vectors for gene cloning in pharmaceuticals, preparation of target genes for genetic engineering drugs, in vitro recombination of target genes with cloning vectors, introduction of recombinant cloning vectors into recipient cells, screening, identification, and analysis of target recombinant clones, and expression of target genes in host cells. The Application part primarily introduces the applications of gene cloning technology in pharmaceuticals, listing various examples of cloning and expression of biopharmaceutical genes, as well as fermentation production examples of various genetic engineering drugs. The content includes: an overview of gene cloning technology in pharmaceuticals, cloning of biopharmaceutical genes and their expression in E. coli, yeast, insects, and animal cells, culture and fermentation of genetic engineering bacteria and cells, separation and purification of genetic engineering drugs, and analysis and testing of genetic engineering drugs. This book combines theory with examples, reflecting cutting-edge science and being highly practical. It is suitable for technical personnel engaged in biotechnology and pharmaceutical research and production, as well as students and teachers in related fields at universities and colleges.