Bio Lab Series -- Application of DNA Molecular Markers in Plant Research

Author: Zhou Yanqing
Publisher:
Publish Date: 2005-05-08
Features: This book primarily elaborates on the concepts, principles, operational procedures, advantages, and disadvantages of DNA molecular marker technologies such as RAPD, RFLP, SCAR, AFLP, SSR, AP-PCR, ISSR, EST, SNP, STS, RBM, SRAP, and TRAP. It also covers key operational points and their applications in plant research, including the construction of genetic linkage maps, genetic diversity analysis, germplasm resource identification, gene localization, phylogenetic relationship analysis, gender identification, gene cloning, gene expression, and molecular marker-assisted selection breeding. The book introduces methods for extracting and purifying DNA from plant genomes, mitochondria, and chloroplasts, as well as detection techniques and operational key points, incorporating the latest research findings and case studies in related fields. Emphasizing the integration of fundamental theories and practical techniques, the book is richly illustrated and combines scientific rigor with accessibility. It focuses on the advanced and systematic presentation of knowledge, reflecting its novel content, comprehensive information, and the interdisciplinary nature of life sciences and their branches. This book is suitable for use and reference by teachers and students in universities and colleges majoring in biological sciences, biotechnology, molecular biology, genetics, molecular ecology and evolution, and cell biology, as well as researchers, technicians, biology teachers in secondary schools, and administrative personnel in relevant fields.