Molecular Diagnostics Laboratory Guide

Author: Qian Shiyun, Editor-in-Chief
Publisher:
Publish Date: 2006-12-01
Features: This book is organized based on the current development of molecular biology testing technologies in recent years and the experimental content of molecular diagnostics offered by medical schools. The book is divided into 5 chapters, covering 35 experiments, and is arranged in the order of nucleic acid isolation and purification, polymerase chain reaction (PCR), nucleic acid identification and analysis, comprehensive genetic engineering experiments, and other related molecular diagnostic technologies. The experiments include basic molecular biology techniques, commonly used molecular biology experiments, and the application of molecular biology techniques in clinical medical laboratory testing. Some practical molecular testing techniques are also detailed in the appendix. Each experiment is structured to include: principle, reagents and equipment, operation steps, result analysis, clinical application, precautions, evaluation, and discussion questions. At the end of the book, there are references, nucleic acid and protein data, introductions to common instruments, the preparation of common reagents and buffers, and a bilingual glossary of common terms. The selected experiments in this book are up-to-date, comprehensive in technology, and highly operational, making it highly readable and practical. This textbook is suitable for undergraduate and graduate students in medical laboratory technology and molecular biology programs in medical schools, as well as for technicians engaged in clinical molecular biology testing.