Fluorescence Analysis (Third Edition)

Author: Xu Jingu
Publisher:
Publish Date: 2006-08-21
Features: This book provides a comprehensive introduction to fluorescence analysis. It elaborates on the basic concepts and principles of fluorescence analysis, the relationship between fluorescence and molecular structure, the effects of environmental factors on fluorescence spectra and intensity, as well as the quenching of solution fluorescence. It introduces the components of fluorescence instruments, the calibration of fluorescence spectra, the sensitivity of fluorescence instruments, and the performance of common instruments on the market. It also covers various fluorescence analysis methods, including conventional fluorescence analysis, synchronous fluorescence analysis, three-dimensional fluorescence spectroscopy analysis, time-resolved and phase-resolved fluorescence analysis, fluorescence polarization determination, low-temperature fluorescence analysis, solid surface fluorescence analysis, kinetic fluorescence analysis, spatially resolved fluorescence analysis techniques, single-molecule fluorescence detection, fluorescence immunoassay, and derivative fluorescence analysis, among others. It provides a brief review of fluorescence analysis for nearly 70 elements and organic compounds such as aliphatic, aromatic, vitamins, amino acids, proteins, nucleic acids, amines, steroids, enzymes, coenzymes, drugs, toxins, and pesticides. This book is rich in content and has a wide range of applications, making it suitable as a textbook or teaching reference for undergraduate and graduate students in relevant fields at universities and colleges. It can also serve as a reference for scientific and technical personnel in research and production departments... The content of this book includes the basic principles of fluorescence analysis, fluorescence analysis methods, and the analysis applications of specific compounds. The third edition has been completely rewritten based on the second edition, with a significant amount of new material added. It includes entirely new sections on spatially resolved fluorescence analysis techniques and single-molecule fluorescence detection, aiming to cover the latest development directions and expand the scope of fluorescence analysis applications.