Biological Separation Engineering (Second Edition)

Author: Sun Yan
Publisher:
Publish Date: 2005-03-01
Features: This book focuses on the separation and purification techniques of biomacromolecules, systematically introducing the fundamental principles, separation operations, process theory, and applications of biomolecule separation and purification. The content is divided into 12 chapters, including Introduction, Cell Separation and Disruption, Primary Separation, Membrane Separation, Extraction, Adsorption Separation Techniques and Theory, Liquid Chromatography, Affinity Chromatography, Electrophoresis and Electrochromatography, Protein Refolding, Crystallization, and Drying. Chapter 2 and Chapter 3 primarily introduce the pretreatment of biological separation processes and primary separation techniques such as precipitation fractionation and foam separation. Chapter 4 introduces various membrane separation methods, their characteristics, and their applications in biological separation. Chapter 5 introduces various extraction methods, particularly the dual-phase extraction and reverse micelle extraction techniques that can be used for the separation and purification of biomacromolecules. Chapters 6 to 10 elaborate on the fundamental principles, characteristics, basic theories, and applications of core techniques in biological separation processes, including adsorption (including ion exchange), chromatography, electrophoresis, electrochromatography, and protein refolding. These chapters include the latest research advancements in the field in recent years and are the core part of the book. The last two chapters introduce the fundamental theories of crystallization and drying and their applications in biological separation. This book is primarily intended as a textbook for undergraduate and graduate students in bioengineering, bioprocess engineering, and other related disciplines at universities and colleges. It can also be used as a reference for researchers, technicians, and management personnel engaged in biotechnology, bioprocess engineering, and biopharmaceutical research.