PCR Technology: A Practical Approach (Second Edition)

Author: (USA) Michael L. Dykstra
Publisher:
Publish Date: 2006-03-01
Features: This book is organized by Cold Spring Harbor Laboratory Press, USA, with experts from internationally authoritative laboratories jointly discussing and contributing to its writing. It is a completely revised edition of the highly acclaimed version published 10 years ago. The book is divided into 8 chapters, introducing the basic principles of PCR methods (including RT-PCR methods), sample preparation, primer design, detection of PCR products, PCR-mediated cloning, preparation of mutants by PCR, differential gene expression analysis using PCR, and PCR methods for RNA samples, among others. At the end of the book, there are convenient and practical appendices and indexes. For fundamental disciplines in life sciences such as molecular biology, biochemistry, genetics, immunology, and cell biology, as well as applied fields like molecular epidemiology, clinical diagnostics, and forensic identification, whether you are a beginner in PCR or an experienced researcher, you will find this manual to be a comprehensive and reliable experimental guide.