PCR Technology: A Practical Approach [Second Edition]

Author: None
Publisher:
Publish Date: 2006-04-07
Features: This book is organized by Cold Spring Harbor Laboratory Press, with experts from internationally authoritative laboratories jointly discussing and contributing to it, representing a completely revised edition of its highly acclaimed version 10 years ago. The book is divided into 8 chapters, introducing the basic principles of PCR methods (including RT-PCR methods), sample preparation, primer design, detection of PCR products, PCR-mediated cloning, preparation of mutants by PCR, and differential gene expression analysis using PCR, as well as PCR methods for RNA samples. At the end of the book, there are practical appendices and an index. For fundamental disciplines in life sciences such as molecular biology, biochemistry, genetics, immunology, and cell biology, as well as applied fields like molecular epidemiology, clinical diagnostics, and forensic identification, whether you are a beginner in PCR or an experienced researcher, you will find this manual to be a detailed and reliable experimental guide.