Principles and Methods of Biochemical Experiments (Second Edition)

Author: Chen Yahui
Publisher:
Publish Date: 2005-08-01
Features: The book is divided into two parts and an appendix. The first part, consisting of fourteen chapters, provides a comprehensive yet concise introduction to the separation, purification, content determination, and purity analysis of biomolecules, with a focus on the fundamental principles of common experimental methods such as chromatography, centrifugation, membrane separation, and electrophoresis. The second part includes 45 selected experiments, covering the separation, preparation, and analysis of biochemical substances such as carbohydrates, lipids, vitamins, proteins, enzymes, and nucleic acids. These experiments involve techniques like paper chromatography, thin-layer chromatography, ion-exchange chromatography, gel filtration chromatography, hydrophobic interaction chromatography, affinity chromatography, metal chelate chromatography, various electrophoretic methods, and immunotechniques, as well as UV-Vis absorption spectroscopy. The book retains some traditional experiments effective for strengthening students' basic experimental methods and skills while introducing newer biochemical techniques. Most experiments can be completed within 6-12 hours, and some can be combined into a comprehensive experiment or conducted independently for flexibility in teaching. Each experiment is followed by discussion questions and reference materials. The appendix includes various commonly used data tables for readers to consult. This book is suitable as a laboratory manual for undergraduate students in biochemistry and related fields at comprehensive universities, normal universities, medical schools, and agricultural and forestry institutions. It can also serve as a reference for teachers and researchers in related disciplines.