How to read a pregnancy test pen?

Patient's question:

Hello doctor, I bought David's pregnancy test pen. Originally, the second row was the urine absorption port, but I put the urine in the first row and it didn't show any reaction. Then I read the instructions and found out that it should be put in the second row. I put the urine in the second row again and it showed no pregnancy. Could it be malfunctioning?

Doctor's answer:

There must be an accuracy issue. Sometimes, if your hormone levels are relatively low, it's not possible for it to be that sensitive. If it's too sensitive, even a slight change would show up, which would also lead to a certain error rate. You can test again in a couple of days.

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