PCR Technology: A Practical Approach - Second Edition

Author: C.W. Deffenbach (USA)
Publisher:
Publish Date: 2006-03-01
Features: This book is organized by Cold Spring Harbor Laboratory Press, with contributions from experts from internationally authoritative laboratories. It is a completely revised edition of the highly acclaimed version published 10 years ago. The book is divided into 8 chapters, covering the basic principles of PCR methods (including RT-PCR methods), sample preparation, primer design, detection of PCR products, PCR-mediated cloning, preparation of mutants by PCR, differential gene expression analysis using PCR, and PCR methods for RNA samples, among others. At the end of the book, there are practical appendices and an index. For fundamental life science disciplines such as molecular biology, biochemistry, genetics, immunology, and cell biology, as well as applied fields like molecular epidemiology, clinical diagnostics, and forensic identification, whether you are a beginner in PCR or an experienced researcher, you will find this manual to be a comprehensive and reliable experimental guide.