Didn't come to, tested and showed negative, what is the reason?

Patient's question:

Detailed medical condition and consultation purpose: Last menstrual period was on 10.23, but it hasn't arrived yet. On 11.30, a pregnancy test was taken and it showed negative. Today, a pregnancy check at the workplace did not detect pregnancy, although the staff mentioned that pregnancy can only be seen after 40 days. There is no sensation of breast tenderness. Please ask the doctor what is going on?
Medical history: Menstrual period was 18 days in July (it started on the second day). Hormone test results: T56.4 ng/mL, E251.8 pg/mL, PRL14.7 ng/mL, PROG3.4 ng/mL, LH11.2 mIU/mL.

Doctor's answer:

Generally, a one-week delay in menstruation is considered normal. If the delay exceeds one week, for women with sexual experience, the first step is to rule out pregnancy. If menstruation is normal, an early pregnancy test can be taken 7 days after the period. It is best to use morning urine because HCG concentration is higher in morning urine. If two red bars appear in the observation area, it indicates pregnancy. If pregnancy is ruled out, it may be caused by menstrual irregularities. Endocrine disorders, psychological factors, gynecological inflammation (such as cervicitis, cervical erosion, endometritis, etc.), or the use of emergency contraception can all cause menstrual irregularities.

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