Practical Electron Microscopy Techniques

Author: Fu Honglan (Compiler)
Publisher:
Publish Date: 2004-07-01
Features: This book consists of 12 chapters and mainly includes 4 parts: Part 1 introduces sample preparation for transmission electron microscopy (TEM), ultrathin sectioning, staining, cryofixation, cryo-substitution, negative staining, and nucleic acid macromolecule spreading techniques; Part 2 covers immunoelectron microscopy; Part 3 describes the structural principles and operational key points of TEM and scanning electron microscopy (SEM), briefly outlining SEM sample preparation methods; Part 4 focuses on laboratory safety in electron microscopy. Additionally, Appendices I and II list common reagent preparation methods and over 70 electron microscopic images. This book is a compilation of the author's 20+ years of experience in TEM technology applications. It serves as both a required or elective textbook for upper-level undergraduate and graduate students in biology departments of comprehensive universities, normal universities, and medical schools, as well as a teaching reference. It is also suitable for researchers engaged in electron microscopy technology.