Principles and Techniques of Protein Engineering

Author: Liu Xianxi et al. (Editor-in-Chief)
Publisher:
Publish Date: 2004-07-19
Features: Protein engineering is a technology that specifically modifies the structure genes of proteins by utilizing gene-directed mutagenesis and gene recombination techniques, based on an understanding of the relationship between protein structure and function, and with the help of bioinformatics knowledge and methods, to produce proteins with new characteristics. This book is written in accordance with the progress of the discipline of protein engineering and the needs of teaching. It consists of ten chapters. Chapter 1 briefly introduces the basic theory, basic content, procedures, applications, and progress of protein engineering; Chapters 2 to 4 introduce the basic principles of protein structure and function, gene recombination, and site-directed mutagenesis technology, respectively; Chapters 5 to 7 introduce the applications of protein engineering in enzyme proteins, antibodies, and the study of peptide drugs; Chapters 8 and 9 introduce the principles and applications of common techniques for protein isolation and purification, as well as structural analysis. Since bioinformatics is often required in protein engineering to analyze the structure and function of proteins and predict structural modifications, Chapter 10 of this book provides a brief introduction to bioinformatics. The purpose of this book is to provide a valuable reference for the training of advanced talents in biology and medicine. From this purpose, the editors have carefully designed the content of the book, systematically introducing the main content of each chapter while highlighting the latest advancements and achievements in this field.