Tissue culture technology and its application in medical research

Author: E Zheng
Publisher:
Publish Date: 2004-08-01
Features: Everyone is eager to read this book and unanimously praises it as an excellent reference. The content is rich and useful for young people, especially graduate students, as a valuable tool book. It is also the best work in the field of tissue culture in China.
— Professor Gao Jin, Basic Research Institute, Chinese Academy of Medical Sciences, Tissue Culture and Molecular Cytology Technology
This is truly a rare gem of a book that one cannot put down after reading.
— Han Zhifang, Xinqiao Hospital, Chongqing
This book is a godsend for us.
— Cheng Naijing and Fang Zhaorong, Shanghai Mental Health Center
The book is very practical and clear in its arrangement, highly readable, and an excellent reference for basic medical workers.
— Liu Xiaomei, Chinese Medical Association
I have benefited greatly from the second edition of your book. I also believe that the millions of readers you have will sincerely thank you for your significant contributions to this cause in China. I deeply appreciate you.
— Peng Jingwu, Hunan Medical University, Tissue Culture and Molecular Cytology Technology
This is an advanced and highly practical book with strong guidance, which has been of immense help to us.
— Xiao Hong, Wuhan
We unanimously agree that the second edition of your edited Tissue Culture Technology is well-written and has been very helpful to us.
— Hu Qing, Molecular Biology Laboratory, Tongji Medical University
This book is a revised version based on the 1995 edition of Tissue Culture and Molecular Cytology Technology. It remains composed of Part I: Cell Culture and Part II: Molecular Cytology. Part I primarily discusses:
(1) Basic theoretical knowledge of cell culture;
(2) Equipment and conditions required for cell culture;
(3) Basic processes and key points of cell culture;
(4) Culture methods for various human tissue cells, including three-dimensional culture techniques for tissue engineering;
(5) Techniques for detecting the biological morphology, shape, and fine structural components of cells;
(6) Practical cell culture techniques in medical and biological research fields.
Part II mainly introduces:
(1) The theoretical foundation of molecular cytology;
(2) Basic molecular biology techniques, including nucleic acid extraction, electrophoresis, labeling, bacterial culture, plasmid extraction, marine pollution water, endonuclease technology, phage packaging, gene cloning, PCR technology, cDNA cloning, and gene libraries, as well as new gene detection and isolation, gene targeting, etc.;
(3) Detection of cellular characteristics and therapeutic gene expression, Southern blotting, transfer blotting, and DNA sequence analysis;
(4) Basic techniques for gene diagnosis and treatment.
Based on the advancements in cell biology technology, this book has undergone significant additions and deletions compared to the 1995 edition, making its content more up-to-date and comprehensive. The book’s features include a combination of theory and technology, high readability, and strong practicality and operability. It includes molecular biology experiment guides, glossary definitions, and various commonly used data, making it highly valuable for clinical physicians and researchers.