Microinjection and Transgenic Experiment Guide

Author: [German] A. Sied-Alliger
Publisher:
Publish Date: 2002-06-01
Features: This book introduces techniques and methods in the fields of microinjection and transgenesis, focusing on experimental strategies, accompanied by detailed references and specific experimental procedures. It covers methods for somatic and embryonic cell injection, with the somatic injection chapter including manual and automated injection systems, post-injection cell imaging microscopy, electron microscopy techniques, and in situ hybridization analysis methods. It provides detailed descriptions of injection experiments for various purposes, such as methods for studying signal transduction and microtubule dynamics. The book primarily elaborates on the principles, new strategies, and diverse applications of transgenic technology, mainly using mice as experimental subjects, with additional use of rabbits and fruit flies. It also introduces knowledge related to mouse housing for transgenic mouse research, as well as the culture of male germ cells and attempts at sperm-mediated transgenesis. It briefly covers strategies for generating various chimeric lines and producing YAC transgenic mice, and discusses the use of mouse embryos as a "test tube" for identifying gene activity and many potential applications. The book also presents various methods for applying tissue markers, the significance of transgenic mouse research for immunology and virology, and knowledge on using inducible site-specific recombinases to alter gene activity. This book is suitable for researchers engaged in functional genomics, transgenesis, and related research, as well as teaching and scientific research personnel in life science-related fields.