Principles and Methods of Biochemical Experiments: Beijing Higher Education Press Excellence Textbook Project · Series Textbooks for Life Sciences Experiments in Higher Education ((Second Edition))

Author: Chen Yahui
Publisher:
Publish Date: 2005-08-01
Features: The book is divided into two parts and an appendix. The first part, consisting of fourteen chapters, provides a comprehensive yet concise introduction to the separation, purification, quantification, and purity analysis of biomolecules, with a focus on the fundamental principles of common experimental methods such as chromatography, centrifugation, membrane separation, and electrophoresis. The second part includes 45 selected experiments, covering the separation, preparation, and analysis of biochemical substances such as carbohydrates, lipids, vitamins, proteins, enzymes, and nucleic acids. These experiments include techniques such as paper chromatography, thin-layer chromatography, ion-exchange chromatography, gel filtration chromatography, hydrophobic interaction chromatography, affinity chromatography, metal chelate chromatography, various electrophoretic and immunotechniques, as well as UV-Vis absorption spectroscopy. The book retains some traditional experiments effective for strengthening students' basic experimental methods and skills while introducing newer biochemical techniques. Most experiments can be completed within 6-12 hours, and some can be combined into a comprehensive experiment or conducted independently for flexibility in teaching. Each experiment is followed by discussion questions and references. The appendix includes various commonly used data tables for readers to consult. This book is suitable for undergraduate students in biochemistry and related fields at comprehensive universities, normal universities, medical schools, and agricultural and forestry colleges as a laboratory manual, as well as for reference by teachers and researchers in related fields.