Principle and Research Application Manual of Flow Cytometry

Author: (Switzerland) Nunez (Nunez/R.) / Liu Bingzi / Xu Zenglu
Publisher:
Publish Date: 2005-08-01
Features: For researchers currently using and those who are about to use flow cytometers, this is a concise basic manual. The book explains the basic principles, basic structure, and data analysis of flow cytometers, provides practical application examples such as molecular hemocytometry and DNA analysis, and is accompanied by rich illustrations, a practical index, and appendices, making it clear and practical. It offers a basic introduction and application guide for researchers new to this field, as well as valuable insights and references for experienced users seeking new application and research areas. This book is part of the "Biological Laboratory Series" publications. Flow cytometry is a high-technology commonly used in life science and medical research for rapid quantitative analysis and sorting of single cells or other biological particles. From a practical perspective, this book concisely introduces the characteristics of new flow cytometers and common data analysis methods, emphasizing the latest application progress in flow cytometry. These applications include traditional fields such as cell ploidy analysis and cell cycle determination, as well as special applications like tumor cell surface feature research, immunophenotyping analysis, and dendritic cell function determination, providing a clear entry guide for beginners. More valuable is the detailed discussion of the latest applications and case studies of flow cytometry in medical clinical research fields such as AIDS prevention and treatment, sperm quantification and motility analysis, parasitic drug reactions, and cytotoxicity research, as well as in cutting-edge life science fields and preclinical trials such as gene transfer efficiency and cytotoxicity evaluation. This greatly expands the application scope of flow cytometry, providing important and practical references for experienced flow cytometer users to explore new research directions. This book is an indispensable guide for researchers, experimental technicians, and university students engaged in life science and medical basic and clinical research in fields such as cell biology, genetics, molecular biology, immunology, hematology, parasitology, andrology, AIDS prevention and treatment, and gene therapy.