Molecular Biology (Revised Edition)

Author: Guo Jinrong
Publisher: Wuhan University Press
Book Description:
The book has been expanded from 16 to 19 chapters. It includes research methods in molecular biology, genomics, and other content. Significant revisions and adjustments have been made to Chapters 1 (Introduction), 6 (Genetic Traits), 10 (Replication of Genetic Material), 12 (Genetic Recombination), 14 (Protein Synthesis), and 16 (Regulation of Eukaryotic Gene Expression). Outdated knowledge and concepts have been removed, while new viewpoints, theories, and concepts, as well as recent research findings and conclusions, have been added. This enhances the richness and advanced nature of the book. The illustrations have been improved, and the structural system of the content is more rational and logically sound. The book can serve as a textbook for universities and colleges in relevant fields, as well as a reference for researchers in related fields.
Table of Contents:
Chapter 1: Introduction
1.1 Definition of Molecular Biology
1.2 Brief Overview of the Development of Molecular Biology
1.3 Main Content of Molecular Biology
1.4 Outlook
Chapter 2: Biological Macromolecules
2.1 Chemical Structure of Biological Macromolecules
2.2 Noncovalent Interactions Determining the Three-Dimensional Structure of Proteins and Nucleic Acids
2.3 Basic Methods for Studying Biological Macromolecules
2.4 Molecular Weight Determination of Biological Macromolecules
Chapter 3: Nucleic Acids
3.1 Basic Structure of DNA
3.2 Basic Properties of DNA
3.3 RNA
3.4 Structural Analysis of Nucleic Acids
Chapter 4: Proteins
4.1 Binding Sites of Proteins and Multisubunit Proteins
4.2 Regulation of Protein Activity
4.3 Important Domains of Proteins
Chapter 5: Interactions Between Biological Macromolecules and Structures of Complex Aggregates
5.1 A Multipeptide Assembleosome—Collagen
5.2 Complex DNA Structures
5.3 Interaction Between DNA and a Protein That Recognizes a Specific Base Sequence
5.4 Biological Membranes
5.5 Self-Assembly of Complex Aggregates
Chapter 6: Genetic Material
6.1 Evidence for Genetic Material
6.2 Properties of Genetic Material
6.3 Genetic Material—RNA
6.4 Genes and Genomes
Chapter 7: Transposable Genetic Elements
7.1 Plasmids
7.2 Transposons
7.3 Viruses and Their Relationship with Plasmids and Transposons
Chapter 8: Methods in Molecular Biology
8.1 Isolation of Biological Macromolecules
8.2 Labeled Tracers
8.3 Nucleic Acid Hybridization
8.4 Mapping and Quantitative Analysis of Transcripts
8.5 In Vivo Determination of Transcription Rate
8.6 Interaction Between DNA and Proteins
Chapter 9: Recombinant DNA Technology—Molecular Cloning
9.1 Vectors and Tools
9.2 Preparation of the Target Gene
9.3 In Vitro Recombination Between the Target Gene and Vector
9.4 Techniques for Introducing Recombinants into Cells
9.5 Screening and Identification of Recombinants
9.6 Expression of Cloned Genes
9.7 Applications of Recombinant DNA Technology
Chapter 10: Replication of Genetic Material
10.1 Overview of Replication
10.2 Proteins Related to DNA Replication
10.3 Initiation, Extension, and Termination of DNA Replication in Prokaryotes
10.4 Replication Process in Eukaryotes
10.5 Regulation of DNA Replication
10.6 Reverse Transcription
10.7 RNA Replication
Chapter 11: DNA Damage Repair and Gene Mutations
11.1 Avoiding Errors in DNA Damage Repair
11.2 Avoiding Errors in DNA Damage Repair and Gene Mutations
11.3 Stress Response (SOS)
11.4 Mutagens, Mutagenesis, Gene Mutations, and Mutants
11.5 Correction of Gene Mutations
Chapter 12: Genetic Recombination
12.1 Mechanism of Homologous Recombination
12.2 Recombination in Bacterial Transformation
12.3 Exchange Between Homologous Double-Stranded DNA Molecules
12.4 Models of Homologous Recombination
12.5 Role of RecA and RecBCD Proteins in Recombination
Chapter 13: Transcription
13.1 Enzymatic Synthesis of RNA
13.2 Types of RNA Molecules and Post-Transcriptional Processing
13.3 Transcription and RNA Processing in Eukaryotes
Chapter 14: Protein Synthesis
14.1 Deciphering the Genetic Code
14.2 Wobble Hypothesis
14.3 Mechanism of Protein Biosynthesis
Chapter 15: Regulation of Prokaryotic Gene Expression
15.1 Lac System and Operon Model
15.2 Galactose Operon
15.3 Trp Operon
15.4 Regulation of Gene Expression in λ Phage
15.5 Regulation of Gene Expression by DNA Rearrangement
15.6 Regulation of Gene Expression by Sigma Factors
15.7 Post-Transcriptional Regulation
Chapter 16: Eukaryotic Genomes and Regulation of Gene Expression
16.1 Eukaryotic Genomes
16.2 Structure of Eukaryotic Genes
16.3 Regulation of Eukaryotic Gene Expression
Chapter 17: Cellular Signaling Regulation
17.1 General Concepts of Cellular Signaling
17.2 Signaling Regulation via G-Protein-Coupled Receptors
17.3 Signaling Regulation via Enzyme-Linked Cell Surface Receptors
17.4 Small Molecule Signaling Regulation
17.5 Cellular Response to Signaling
Chapter 18: Molecular Biology of Cancer
18.1 Molecular Basis of Cancer—DNA Sequence Alterations
18.2 Cancer Development Involves the Synergistic Action of Multiple Factors
18.3 Proto-oncogenes and Oncogenes
18.4 Activation of Proto-oncogenes
18.5 Tumor Suppressor Proteins
Chapter 19: Genomics
19.1 Sequencing of Genomes
19.2 Applications of Genomics