Application of DNA molecular marker technology in plant research

Author: Zhou Yanqing
Publisher:
Publish Date: 2005-05-01
Features: This book primarily elaborates on the concepts, principles, operational procedures, advantages and disadvantages, key points of operation, and applications of various DNA molecular marker technologies such as RAPD, RFLP, SCAR, AFLP, SSR, AP-PCR, ISSR, EST, SNP, STS, RBM, SRAP, and TRAP in plant research. It covers the construction of genetic linkage maps, genetic diversity analysis, germplasm resource identification, gene localization, phylogenetic relationship analysis, gender identification, gene cloning, gene expression, and molecular marker-assisted selection breeding. The book introduces the extraction, purification, detection methods, and key points of operation for plant genomic DNA, mitochondrial DNA, and chloroplast DNA, incorporating the latest research findings and case studies in related fields. It emphasizes the integration of fundamental theories and practical techniques, presented with illustrations and diagrams. The book focuses on scientific accuracy and accessibility, as well as the advancement and systematic nature of the knowledge. It reflects the characteristics of novel content, comprehensive information, and the interdisciplinary integration of life sciences and their branches. This book is suitable for use and reference by teachers and students in universities and colleges majoring in biology, biotechnology, molecular biology, genetics, molecular ecology and evolution, and cell biology, as well as researchers, technicians, biology teachers in secondary schools, and administrative personnel in relevant fields.