Molecular Cloning Manual: Protein-Protein Interaction

Author: Golemis
Publisher:
Publish Date: 2004-10-01
Features: Research on protein-protein interactions not only reveals the functions of proteins at the molecular level but is also crucial for understanding the patterns of life processes such as growth, development, differentiation, and apoptosis. It provides an important theoretical foundation for exploring the mechanisms of major diseases, disease treatment, disease prevention, and new drug development. This book consists of 35 chapters. It first introduces issues such as signal transduction and human genetics/drug genomics, emphasizing the key role of protein-protein interactions in these processes. Then, it is divided into three parts to cover the following content: 6 methods for analyzing protein-protein interactions established based on standard molecular biology, biochemistry, and microbiology techniques; 6 biophysical methods for identifying and detecting protein-protein interactions; recently improved techniques related to GST precipitation and immunoprecipitation, two complement systems, incremental truncation methods, protein bundling, and high-throughput methods. Finally, it discusses the integration of protein-protein interaction data and model construction, as well as the clinical applications of these models. This book is comprehensive and detailed, providing a practical guide for key techniques in protein-protein interaction research. It also offers a wealth of techniques and reviews for identifying and manipulating proteins, covering the most complete and effective methods for analyzing protein-protein interactions to date. From theoretical explanations to the listing and comparison of relevant protocols, it includes classical molecular biology, biochemistry, and microbiology methods, biophysical methods, computational analysis, and the construction of interaction models. The book is systematic, easy to navigate, and serves as an excellent reference for life science researchers.