Proteomics: From Sequence to Function

Author: [UK] S.R. Pennington M.J. Dunn
Publisher:
Publish Date: 2002-09-01
Features: The development of protein identification technology and the implementation and subsequent completion of genome sequencing projects have led to the emergence and advancement of proteomics. Proteomics is a science that studies the expression and function of proteins based on genomics. It is built upon the integration of various experimental methods, including gene function analysis based on cDNA array mRNA expression profiles, genome-wide yeast two-hybrid systems, protein-protein interaction analysis, as well as protein expression, sequencing, and structural analysis. This book focuses on the following topics: the relationship between genomics and proteomics; current research methods for mRNA expression profiles; proteomics research methods, such as two-dimensional polyacrylamide gel electrophoresis, protein detection, mass spectrometry methods for protein identification, and advancements in mass spectrometry and image analysis methods for protein expression quantification; automation in proteomics research; the application of proteomics in drug development; the use of phage antibodies in proteomics research; glycomics and proteomics; establishing a proteomics technology system in academic laboratory settings; and the application of proteomics in plant genetics and breeding. This book is suitable for faculty and students in life science and medical disciplines at universities, as well as R&D personnel in the biotechnology industry.