Application of gene cloning technology in pharmaceuticals

Author: Yang Rude
Publisher:
Publish Date: 2004-01-01
Features: This book combines theory with examples, reflecting cutting-edge science and being highly practical. It is suitable for technical personnel engaged in biotechnology and pharmaceutical research and production, as well as students and teachers in related fields at universities and colleges. The book discusses the basic principles of gene cloning technology and its research and applications in biopharmaceuticals. The book is divided into two parts: Technology and Application. The Technology Part emphasizes systematic explanations of the fundamental knowledge of gene cloning technology, including: an overview of gene cloning technology, commonly used tools for gene cloning in pharmaceuticals, commonly used plasmid and phage vectors in gene cloning technology for pharmaceuticals, the production of target genes for genetic engineering drugs, in vitro recombination of target genes with cloning vectors, introduction of recombinant cloning vectors into recipient cells, screening, identification, and analysis of target recombinant clones, and the expression of target genes in host cells. The Application Part primarily introduces the applications of gene cloning technology in pharmaceuticals, providing examples of the cloning and expression of various biopharmaceutical genes, as well as fermentation production examples of various genetic engineering drugs. The content includes: an overview of gene cloning technology in pharmaceuticals, the cloning of biopharmaceutical genes and their expression in E. coli, yeast, insect, and animal cells, the culture and fermentation of genetic engineering bacteria and cells, the separation and purification of genetic engineering drugs, and the analysis and testing of genetic engineering drugs.