Tissue culture and molecular cytology techniques

Author: E Zheng
Publisher:
Publish Date: 2001-01-01
Features: This book consists of Part I: Cell Culture and Part II: Molecular Cytology Techniques, which are interconnected in content.
Part I systematically introduces tissue culture techniques, with the main content including: ① the theoretical foundation of cell differentiation, growth, and proliferation in vitro, as well as the survival conditions of cells outside the body; ② general principles and basic processes of cell culture methods; ③ the culture methods of individual tissue cells; ④ various research techniques for in vitro cultured cells.
Part II focuses on methods for studying cells using applied molecular biology techniques, including: ① the theoretical foundation of molecular cytology techniques; ② a comprehensive introduction to commonly used basic molecular cytology techniques such as nucleic acid extraction and separation, electrophoresis, radioactive isotope labeling, bacterial culture, endonuclease technology, plasmid extraction, and phage culture and packaging; ③ cell genetic engineering; ④ the detection of genetic properties and expression of cultured cells, including common techniques such as blot transfer, RFLP detection, and DNA sequence analysis; ⑤ the application of molecular cytology techniques, including gene resistance detection and techniques related to gene diagnosis and treatment.
The book is rich in content, up-to-date in information, highlights key points, and closely integrates theory and technology. It also includes a comprehensive appendix at the end, featuring glossary definitions and various laboratory data. The chapter arrangement is logical, well-structured, clear, and accompanied by concise illustrations. The narrative is easy to understand and highly practical. Therefore, this book is not only suitable for young researchers, graduate students, clinical doctors, and high-level researchers in medical fields but also holds significant reference value for senior researchers.