Principles and Applications of Biochemical Technology

Author: Zhao Yongfang
Publisher:
Publish Date: 2002-07-01
Features: This book consists of twenty chapters and is divided into three parts: Part I outlines the preparation procedures and basic principles of preparing biomacromolecules such as proteins and nucleic acids; Part II explains common methods for isolating these substances from animal, plant, and microbial materials, such as ion exchange chromatography, hydrophobic chromatography, affinity chromatography, isoelectric focusing, gel filtration, and high-performance reversed-phase liquid chromatography; Part III introduces related methods for identifying biomacromolecules, including isotope labeling (including labeling of DNA, RNA, and proteins), genetic recombination, DNA sequencing, biochips, apoptosis, biosensors, various electrophoresis techniques (including gel electrophoresis, isoelectric focusing electrophoresis, agar electrophoresis, transfer electrophoresis, capillary electrophoresis, and the preparation of staining solutions used after electrophoresis of various biomacromolecules), immunoassays (including the preparation of monoclonal antibodies, immunodiffusion, various immunoelectrophoresis techniques, microsphere assays, solid-phase immunoassays, thin-layer chromatography, and gas chromatography). While elucidating the basic principles of each method, the book also discusses major operations and application examples. At the end of each chapter, there are review questions and references, with a total of over 370 illustrations and tables throughout the book.