Molecular Cloning Strategies for Targeting New Genes: Theory and Methods

Author: Zhang Xiumin Wang Yiqiang
Publisher:
Publish Date: 1999-07-01
Features: This book introduces the main strategies and advancements in molecular cloning for finding new genes, divided into two parts: theoretical and technical methods, covering a total of seven chapters: PCR program and condition optimization; cloning of PCR products; mutation, recombination, and in vitro selection; cloning of adjacent unknown DNA; library construction and screening; cDNA analysis, cloning, and their applications in differential and subtractive techniques; cloning of gene family members. The theoretical part is clear and concise, highly targeted, and covers the main aspects of molecular cloning, helping readers master molecular cloning strategies for different genes. The technical methods section is systematic and detailed, making it easy to operate. The greatest feature of this book is that, in addition to the theoretical discussions dedicated to each chapter, every specific method or step includes theoretical summaries and explanations of potential difficulties and confusions encountered in experiments, providing theoretical analysis and guidance on troubleshooting for experimental failures. Unlike general PCR-related books on molecular biology experimental guides in China, this book provides in-depth discussions on the theory and methods of PCR molecular cloning strategies. It can serve as both an introductory guide for beginners and a working manual for experts, suitable for researchers and educators engaged in basic and applied research in biology, medicine, and other life science-related fields, including graduate students (medicine, pharmacy, agriculture, forestry, animal husbandry, natural sciences, engineering).