High-throughput screening of bioactive components

Author: Qin Lüping
Publisher:
Publish Date: 2002-01-01
Features:
The first part is about biodiversity and natural active ingredients in HTS, introducing the relationship between biodiversity and HTS in terrestrial plants, microorganisms, marine organisms, etc.
The second part is about combinatorial chemistry and HTS. Combinatorial chemistry provides a large number of screening compounds for HTS and has gradually become a conventional organic compound synthesis technique.
The third part introduces the analytical methods and detection techniques of HTS, including Affinity Scintillation Assay (SPA), scintillation plate technology, detection methods for neurotrophic factor receptor small molecule agonists and antagonists, homogeneous time-resolved fluorescence (HTR), fluorescence polarization, reporter gene detection methods, PCR quantitative methods based on gene expression screening, and high-efficiency microphysiological function detection methods. It also introduces the application of the optical sensor BLAcore in bioactivity screening.
The fourth part is about the automation and data management of HTS laboratories, mainly focusing on the design and operation of laboratories, automation and data management, and the application of robotic technology.